Differential expression of genes involved in alternative glycolytic pathways, phosphorus scavenging and recycling in response to aluminum and phosphorus interactions in Citrus roots.
Identifieur interne : 001356 ( Main/Exploration ); précédent : 001355; suivant : 001357Differential expression of genes involved in alternative glycolytic pathways, phosphorus scavenging and recycling in response to aluminum and phosphorus interactions in Citrus roots.
Auteurs : Lin-Tong Yang [République populaire de Chine] ; Huan-Xin Jiang ; Yi-Ping Qi ; Li-Song ChenSource :
- Molecular biology reports [ 1573-4978 ] ; 2012.
English descriptors
- KwdEn :
- Aluminum (metabolism), Citric Acid (metabolism), Citrus (genetics), Citrus (metabolism), DNA, Complementary (isolation & purification), Gene Expression Regulation, Plant, Genes, Plant (genetics), Glycolysis (genetics), Malates (metabolism), Molecular Sequence Data, Phosphorus (metabolism), Plant Leaves (metabolism), Plant Roots (metabolism), Plant Shoots (growth & development), Plant Stems (metabolism), Reverse Transcriptase Polymerase Chain Reaction, Seedlings (growth & development), Seedlings (metabolism).
- MESH :
- chemical , isolation & purification : DNA, Complementary.
- chemical , metabolism : Aluminum, Citric Acid, Malates, Phosphorus.
- genetics : Citrus, Genes, Plant, Glycolysis.
- growth & development : Plant Shoots, Seedlings.
- metabolism : Citrus, Plant Leaves, Plant Roots, Plant Stems, Seedlings.
- Gene Expression Regulation, Plant, Molecular Sequence Data, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
The objective was to determine the possible links between the expression levels of genes involved in alternative glycolytic pathways, phosphorus (P) scavenging and recycling and Citrus tolerance to aluminum (Al) and/or P-deficiency. 'Xuegan' (Citrus sinensis) and 'Sour pummelo' (Citrus grandis) seedlings were irrigated for 18 weeks with nutrient solution containing 0 and 1.2 mM AlCl(3)·6H(2)O × 0, 50 and 200 μM KH(2)PO(4). C. sinensis displayed more tolerant to Al and P-deficiency than C. grandis. Under Al stress, C. sinensis accumulated more Al in roots and less Al in shoots than C. grandis. P concentration was higher in C. sinensis shoots and roots than in C. grandis ones. C. sinensis roots secreted more malate and citrate than C. grandis ones when exposed to Al. Al-induced-secretion of malate and citrate by excised roots from Al-treated seedlings decreased with increasing P supply. Al-induced-secretion of malate and citrate from roots and Al precipitation by P in roots might be responsible for Al-tolerance of C. sinensis. qRT-PCR analysis showed that Al-activated malate transporter (ALMT1), ATP-dependent phosphofructokinase (ATP-PFK), pyrophosphate-dependent phosphofructokinase (PPi-PFK), tonoplast adenosine-triphosphatase subunit A (V-ATPase A), tonoplast pyrophosphatase (V-PPiase), pyruvate kinase (PK), acid phosphatase (APase), phosphoenolpyruvate carboxylase (PEPC), malic enzyme (ME) and malate dehydrogenase (MDH) genes might contribute to the tolerance of Citrus to Al and/or P-deficiency, but any single gene could not explain the differences between the two species. Citrus tolerance to Al and/or P-deficiency might be caused by the coordinated regulation of gene expression involved in alternative glycolytic pathways, P scavenging and recycling.
DOI: 10.1007/s11033-012-1457-7
PubMed: 22307782
Affiliations:
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last="Jiang">Huan-Xin Jiang</name></author><author><name sortKey="Qi, Yi Ping" sort="Qi, Yi Ping" uniqKey="Qi Y" first="Yi-Ping" last="Qi">Yi-Ping Qi</name></author><author><name sortKey="Chen, Li Song" sort="Chen, Li Song" uniqKey="Chen L" first="Li-Song" last="Chen">Li-Song Chen</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22307782</idno><idno type="pmid">22307782</idno><idno type="doi">10.1007/s11033-012-1457-7</idno><idno type="wicri:Area/PubMed/Corpus">000620</idno><idno type="wicri:Area/PubMed/Curation">000620</idno><idno type="wicri:Area/PubMed/Checkpoint">000620</idno><idno type="wicri:Area/Ncbi/Merge">000F04</idno><idno type="wicri:Area/Ncbi/Curation">000F04</idno><idno type="wicri:Area/Ncbi/Checkpoint">000F04</idno><idno type="wicri:Area/Main/Merge">001371</idno><idno type="wicri:Area/Main/Curation">001356</idno><idno 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sort="Jiang, Huan Xin" uniqKey="Jiang H" first="Huan-Xin" last="Jiang">Huan-Xin Jiang</name></author><author><name sortKey="Qi, Yi Ping" sort="Qi, Yi Ping" uniqKey="Qi Y" first="Yi-Ping" last="Qi">Yi-Ping Qi</name></author><author><name sortKey="Chen, Li Song" sort="Chen, Li Song" uniqKey="Chen L" first="Li-Song" last="Chen">Li-Song Chen</name></author></analytic><series><title level="j">Molecular biology reports</title><idno type="eISSN">1573-4978</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aluminum (metabolism)</term><term>Citric Acid (metabolism)</term><term>Citrus (genetics)</term><term>Citrus (metabolism)</term><term>DNA, Complementary (isolation & purification)</term><term>Gene Expression Regulation, Plant</term><term>Genes, Plant (genetics)</term><term>Glycolysis (genetics)</term><term>Malates (metabolism)</term><term>Molecular Sequence Data</term><term>Phosphorus (metabolism)</term><term>Plant Leaves (metabolism)</term><term>Plant Roots (metabolism)</term><term>Plant Shoots (growth & development)</term><term>Plant Stems (metabolism)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Seedlings (growth & development)</term><term>Seedlings (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>DNA, Complementary</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Aluminum</term><term>Citric Acid</term><term>Malates</term><term>Phosphorus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Citrus</term><term>Genes, Plant</term><term>Glycolysis</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Plant Shoots</term><term>Seedlings</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Citrus</term><term>Plant Leaves</term><term>Plant Roots</term><term>Plant Stems</term><term>Seedlings</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Molecular Sequence Data</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The objective was to determine the possible links between the expression levels of genes involved in alternative glycolytic pathways, phosphorus (P) scavenging and recycling and Citrus tolerance to aluminum (Al) and/or P-deficiency. 'Xuegan' (Citrus sinensis) and 'Sour pummelo' (Citrus grandis) seedlings were irrigated for 18 weeks with nutrient solution containing 0 and 1.2 mM AlCl(3)·6H(2)O × 0, 50 and 200 μM KH(2)PO(4). C. sinensis displayed more tolerant to Al and P-deficiency than C. grandis. Under Al stress, C. sinensis accumulated more Al in roots and less Al in shoots than C. grandis. P concentration was higher in C. sinensis shoots and roots than in C. grandis ones. C. sinensis roots secreted more malate and citrate than C. grandis ones when exposed to Al. Al-induced-secretion of malate and citrate by excised roots from Al-treated seedlings decreased with increasing P supply. Al-induced-secretion of malate and citrate from roots and Al precipitation by P in roots might be responsible for Al-tolerance of C. sinensis. qRT-PCR analysis showed that Al-activated malate transporter (ALMT1), ATP-dependent phosphofructokinase (ATP-PFK), pyrophosphate-dependent phosphofructokinase (PPi-PFK), tonoplast adenosine-triphosphatase subunit A (V-ATPase A), tonoplast pyrophosphatase (V-PPiase), pyruvate kinase (PK), acid phosphatase (APase), phosphoenolpyruvate carboxylase (PEPC), malic enzyme (ME) and malate dehydrogenase (MDH) genes might contribute to the tolerance of Citrus to Al and/or P-deficiency, but any single gene could not explain the differences between the two species. Citrus tolerance to Al and/or P-deficiency might be caused by the coordinated regulation of gene expression involved in alternative glycolytic pathways, P scavenging and recycling.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Fujian</li></region><settlement><li>Fuzhou</li></settlement></list><tree><noCountry><name sortKey="Chen, Li Song" sort="Chen, Li Song" uniqKey="Chen L" first="Li-Song" last="Chen">Li-Song Chen</name><name sortKey="Jiang, Huan Xin" sort="Jiang, Huan Xin" uniqKey="Jiang H" first="Huan-Xin" last="Jiang">Huan-Xin Jiang</name><name sortKey="Qi, Yi Ping" sort="Qi, Yi Ping" uniqKey="Qi Y" first="Yi-Ping" last="Qi">Yi-Ping Qi</name></noCountry><country name="République populaire de Chine"><region name="Fujian"><name sortKey="Yang, Lin Tong" sort="Yang, Lin Tong" uniqKey="Yang L" first="Lin-Tong" last="Yang">Lin-Tong Yang</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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